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- <meta content="Harlow, Lucy D." name="eprints.creators_name" />
- <meta content="Koutoulis, Anthony" name="eprints.creators_name" />
- <meta content="Hallegraeff, Gustaaf M." name="eprints.creators_name" />
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- <meta content="Paralytic shellfish poisoning toxins (PSTs) such as saxitoxin are believed to be synthesised from precursor molecules including
- arginine, S-adenosylmethionine (SAM) and acetate. The enzyme SAM synthetase is a good candidate as an early
- acting enzyme in the PST biosynthetic pathway. We have used degenerate PCR to identify the gene encoding SAM synthetase,
- Sam, in dinoflagellates. Several different PCR products were cloned and sequenced from PST-producing strains of
- the dinoflagellates Alexandrium minutum, A. catenella and Gymnodinium catenatum and primers specific to dinoflagellate
- Sam used for further sequence analysis in 11 species of toxic and nontoxic dinoflagellate genera, including: Alexandrium,
- Gymnodinium, Karenia, Karlodinium, Noctiluca, Prorocentrum and Takayama. At the nucleotide level, Sam clones were
- unique to dinoflagellates and the most commonly identified Sam was highly conserved between dinoflagellate species.
- Dinoflagellate Sam G C content was both typical (60.8%) or lower (40.7%) compared to A. tamarense. The derived
- protein sequences showed a low and equal level of similarity to Sam from other species representing a range of Kingdoms.
- DNA sequence information for dinoflagellate Sam provides important insights for dinoflagellate codon usage, which will
- facilitate primer design to target novel dinoflagellate genes." name="eprints.abstract" />
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- <meta content="Paralytic shellfish poisoning toxins (PSTs) such as saxitoxin are believed to be synthesised from precursor molecules including
- arginine, S-adenosylmethionine (SAM) and acetate. The enzyme SAM synthetase is a good candidate as an early
- acting enzyme in the PST biosynthetic pathway. We have used degenerate PCR to identify the gene encoding SAM synthetase,
- Sam, in dinoflagellates. Several different PCR products were cloned and sequenced from PST-producing strains of
- the dinoflagellates Alexandrium minutum, A. catenella and Gymnodinium catenatum and primers specific to dinoflagellate
- Sam used for further sequence analysis in 11 species of toxic and nontoxic dinoflagellate genera, including: Alexandrium,
- Gymnodinium, Karenia, Karlodinium, Noctiluca, Prorocentrum and Takayama. At the nucleotide level, Sam clones were
- unique to dinoflagellates and the most commonly identified Sam was highly conserved between dinoflagellate species.
- Dinoflagellate Sam G C content was both typical (60.8%) or lower (40.7%) compared to A. tamarense. The derived
- protein sequences showed a low and equal level of similarity to Sam from other species representing a range of Kingdoms.
- DNA sequence information for dinoflagellate Sam provides important insights for dinoflagellate codon usage, which will
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- <h1 class="ep_tm_pagetitle">S-adenosylmethionine synthetase genes from eleven marine dinoflagellates</h1>
- <p style="margin-bottom: 1em" class="not_ep_block"><span class="person_name">Harlow, Lucy D.</span> and <span class="person_name">Koutoulis, Anthony</span> and <span class="person_name">Hallegraeff, Gustaaf M.</span> (2007) <xhtml:em>S-adenosylmethionine synthetase genes from eleven marine dinoflagellates.</xhtml:em> Phycologia, 46 . pp. 46-53.</p><p style="margin-bottom: 1em" class="not_ep_block"></p><table style="margin-bottom: 1em" class="not_ep_block"><tr><td valign="top" style="text-align:center"><a href="http://eprints.utas.edu.au/1675/1/Harlow_etal_2007.pdf"><img alt="[img]" src="http://eprints.utas.edu.au/style/images/fileicons/application_pdf.png" border="0" class="ep_doc_icon" /></a></td><td valign="top"><a href="http://eprints.utas.edu.au/1675/1/Harlow_etal_2007.pdf"><span class="ep_document_citation">PDF</span></a> - Full text restricted - Requires a PDF viewer<br />400Kb</td><td><form method="get" accept-charset="utf-8" action="http://eprints.utas.edu.au/cgi/request_doc"><input value="2164" name="docid" accept-charset="utf-8" type="hidden" /><div class=""><input value="Request a copy" name="_action_null" class="ep_form_action_button" onclick="return EPJS_button_pushed( '_action_null' )" type="submit" /> </div></form></td></tr></table><p style="margin-bottom: 1em" class="not_ep_block">Official URL: <a href="http://dx.doi.org/10.2216/06-28.1">http://dx.doi.org/10.2216/06-28.1</a></p><div class="not_ep_block"><h2>Abstract</h2><p style="padding-bottom: 16px; text-align: left; margin: 1em auto 0em auto">Paralytic shellfish poisoning toxins (PSTs) such as saxitoxin are believed to be synthesised from precursor molecules including
- arginine, S-adenosylmethionine (SAM) and acetate. The enzyme SAM synthetase is a good candidate as an early
- acting enzyme in the PST biosynthetic pathway. We have used degenerate PCR to identify the gene encoding SAM synthetase,
- Sam, in dinoflagellates. Several different PCR products were cloned and sequenced from PST-producing strains of
- the dinoflagellates Alexandrium minutum, A. catenella and Gymnodinium catenatum and primers specific to dinoflagellate
- Sam used for further sequence analysis in 11 species of toxic and nontoxic dinoflagellate genera, including: Alexandrium,
- Gymnodinium, Karenia, Karlodinium, Noctiluca, Prorocentrum and Takayama. At the nucleotide level, Sam clones were
- unique to dinoflagellates and the most commonly identified Sam was highly conserved between dinoflagellate species.
- Dinoflagellate Sam G C content was both typical (60.8%) or lower (40.7%) compared to A. tamarense. The derived
- protein sequences showed a low and equal level of similarity to Sam from other species representing a range of Kingdoms.
- DNA sequence information for dinoflagellate Sam provides important insights for dinoflagellate codon usage, which will
- facilitate primer design to target novel dinoflagellate genes.</p></div><table style="margin-bottom: 1em" border="0" cellpadding="3" class="not_ep_block"><tr><th valign="top" class="ep_row">Item Type:</th><td valign="top" class="ep_row">Article</td></tr><tr><th valign="top" class="ep_row">Additional Information:</th><td valign="top" class="ep_row">The definitive version is available at www.blackwell-synergy.com</td></tr><tr><th valign="top" class="ep_row">Keywords:</th><td valign="top" class="ep_row">Alexandrium catenella, Alexandrium minutum, Gymnodinium catenatum, Dinoflagellate, S-adenosylmethionine
- synthetase, Neurotoxin, PCR</td></tr><tr><th valign="top" class="ep_row">Subjects:</th><td valign="top" class="ep_row"><a href="http://eprints.utas.edu.au/view/subjects/270400.html">270000 Biological Sciences > 270400 Botany</a></td></tr><tr><th valign="top" class="ep_row">Collections:</th><td valign="top" class="ep_row">UNSPECIFIED</td></tr><tr><th valign="top" class="ep_row">ID Code:</th><td valign="top" class="ep_row">1675</td></tr><tr><th valign="top" class="ep_row">Deposited By:</th><td valign="top" class="ep_row"><span class="ep_name_citation"><span class="person_name">Assoc Prof Anthony Koutoulis</span></span></td></tr><tr><th valign="top" class="ep_row">Deposited On:</th><td valign="top" class="ep_row">27 Aug 2007</td></tr><tr><th valign="top" class="ep_row">Last Modified:</th><td valign="top" class="ep_row">11 Feb 2008 10:53</td></tr><tr><th valign="top" class="ep_row">ePrint Statistics:</th><td valign="top" class="ep_row"><a target="ePrintStats" href="/es/index.php?action=show_detail_eprint;id=1675;">View statistics for this ePrint</a></td></tr></table><p align="right">Repository Staff Only: <a href="http://eprints.utas.edu.au/cgi/users/home?screen=EPrint::View&eprintid=1675">item control page</a></p>
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